Figure 1From: Increased proteasome activator 28 gamma (PA28γ) levels are unspecific but correlate with disease activity in rheumatoid arthritis Preparation of recombinant PA28γ and rabbit polyclonal antibodies to PA28γ. A) Coomassie stained SDS gel of purified recombinant PA28γ. Supernatant 1 and 2 (SN1 and SN2) contain the soluble E. coli proteins after the first two solubilization steps. Pelleted His-PA28γ precipitate was solved in 8 M urea. After dialysis insoluble His-PA28γ was removed by centrifugation (Pdia) and soluble recombinant protein (RP) was stored at 4°C. B) Immunoblot analysis with anti-PA28γ polyclonal antibody (crude serum, eluate of Protein A purification and eluate of PA28γ specific peptide column). Cellular extract of carcinoma cells (CE, 30 μg) and recombinant His-PA28γ protein (RP, 1 μg) were separated by SDS-PAGE, blotted and incubated with rabbit anti-PA28γ antibody purifications.Back to article page