Lack of association between the CHL1 gene and adolescent idiopathic scoliosis susceptibility in Han Chinese: a case-control study

Table 1 Primers and conditions of PCR-RFLP analysis

SNPs	Primer Sequence 5′-3′	Annealing temperature (C)	PCR size (bp)	Enzyme	Restriction fragment size (bp)
rs2272522	F:AAGAGGACTAATCGTATATCTAATGGTC R: TGAAATTCCATTAATAGGCACTGA	57	110	AvaII	C:25 + 85
rs2272522	F:AAGAGGACTAATCGTATATCTAATGGTC R: TGAAATTCCATTAATAGGCACTGA	57	110	AvaII	T:110
rs331894	F: CAGAAACGACCCAGTAGACTCC	59	113	MnlI	A:49 + 37 + 17 + 10
	R: GTTTTCTTGCCCTCCCTTTC				A:49 + 37 + 17 + 10
	R: GTTTTCTTGCCCTCCCTTTC				G:86 + 17 + 10
rs2055314	F: TCCATCCCATGCACATCTTA	59	492	HindIII	C:492
	R: TGTCCGTCCACACATGCTAT				C:492
	R: TGTCCGTCCACACATGCTAT				T:227 + 245
rs2272524	F:AAGGTTTTCTTTGTATTTTACTTTGC	59	106	HhaI	G:27 + 79
rs2272524	R: TCACCACCAATTTTGTTCCA	59	106	HhaI	G:27 + 79
					A:106

ISSN: 1471-2474