SNPs | Primer Sequence 5′-3′ | Annealing temperature (C) | PCR size (bp) | Enzyme | Restriction fragment size (bp) |
---|---|---|---|---|---|
rs2272522 | F:AAGAGGACTAATCGTATATCTAATGGTC R: TGAAATTCCATTAATAGGCACTGA | 57 | 110 | AvaII | C:25 + 85 |
T:110 | |||||
rs331894 | F: CAGAAACGACCCAGTAGACTCC | 59 | 113 | MnlI | A:49 + 37 + 17 + 10 |
R: GTTTTCTTGCCCTCCCTTTC | |||||
G:86 + 17 + 10 | |||||
rs2055314 | F: TCCATCCCATGCACATCTTA | 59 | 492 | HindIII | C:492 |
R: TGTCCGTCCACACATGCTAT | |||||
T:227 + 245 | |||||
rs2272524 | F:AAGGTTTTCTTTGTATTTTACTTTGC | 59 | 106 | HhaI | G:27 + 79 |
R: TCACCACCAATTTTGTTCCA | |||||
A:106 |