Figure 1From: Influence of osteogenic stimulation and VEGF treatment on in vivo bone formation in hMSC-seeded cancellous bone scaffolds Experimental setup. In total 12 athymic nude mice ranging from six to eight weeks of age were used for this study (a). According to the seeding and culture conditions, 4 groups were built: 1. blank scaffolds, 2. seeded with hMSCs, 3. seeded with osteogenically stimulated hMSCs, 4. seeded with osteogenically stimulated hMSCs and treated with VEGF. After 2 and 12 weeks, scaffolds were harvested including the surrounding tissue. One scaffold of each group was split into two parts (b): one half was destined for decalcification the other for un-decalcified histological examination. The other scaffold of each group was shock frozen in fluid nitrogen and stored at -80°C until use for molecular analysis (b).Back to article page