Table 2 Sequences of primers used in the real-time PCR
From: In vivo experimental intervertebral disc degeneration induced by bleomycin in the rhesus monkey
Name | GeneBank accession No. | Sequence (5′-3′) | |
|---|---|---|---|
GAPDH | XM_001091567.2 | Forward | ACAATCCCATCACCATCTCGC |
Reverse | GACCCTTTTGGCCTCCTCATC | ||
Aggrecan | XM_002804944.1 | Forward | ACTCGCTGAGTGTCAGCATC |
Reverse | ACACACGGCTCCACTTGATT | ||
Col1α1 | XM_001096194.2 | Forward | CCAGCCGCAAAGAGTCTACA |
Reverse | TGGTGGGATGTCTTCGTCTTG | ||
Col2α1 | XM_001100559.2 | Forward | GTGTCAGGGCCAGGATGTC |
Reverse | AGGGGCACCTTTTTCACCTT | ||
MMP-3 | XM_001098400.2 | Forward | GGCGCAAATCTCTCAGGAAG |
Reverse | GGCCCAGAACTGATTTCCTTT | ||
ADAMTS-5 | XM_001103439.2 | Forward | GTGGCTCACGAAATCGGACA |
Reverse | GGTGGCTGAAGTGCATTTGG | ||
vWF | NM_001243086.1 | Forward | GAGGGTGGTTGGTGGATGTC |
Reverse | CTAGGCCATGCTCCTAGCTG | ||
TNF-α | NM_001047149 | Forward | CCCCAAGGACCCCTCTCTAA |
Reverse | GGGTTTGCTACAACATGGGC | ||
IL-1β | NM_001042756 | Forward | GACGTCGATGGCCCTAAACA |
Reverse | AAGCCCTCGTTGTAGTGCTC | ||
IL-6 | NM_001042733 | Forward | GGTACATCCTCGACGGCATC |
Reverse | CCAGGCAAGTGTCCTCATTG | ||
Caveolin 1 | NM_001168614 | Forward | ACGTAGACTCGGAGGGACAT |
Reverse | AGCGATGGTGATTCCCCAAG | ||
TGF β1 | XM_001100842 | Forward | GGGCTACCATGCCAACTTCT |
Reverse | CCAGGACCTTGCTGTACTGT |