Figure 4

Osteogenic, adipogenic and chondrogenic differentiation results. a) Alizarin red contents per protein at days 3, 7, 14 and 21 are displayed in the right diagram, ALP contents per protein per ml and min at days 3, 7, 14 and 21 are displayed in the left diagram. No significant differences between fluorescence or magnetic sorting and the control MSCs could be detected. b) No differences regarding adipogenic differentiation of MSCs cultured in DMEM or ES medium and sorted for CD146 by fluorescence cytometry (DMEM-FACS and ES-FACS) or magnetically (DMEM-MACS and ES-MACS) could be detected. Light microscopy, magnification x100. c) Mean GAG/DNA content of control MSCs, FACS and MACS sorted cells after 21 days of chondrogenic differentiation is displayed. *p < 0.05.