Figure 3

Surface marker distribution on MSCs. a) MSCs were clearly negative for CD14 and CD34 while CD45 was expressed on some of the cells. MSCs were positive for CD73, CD90 and CD105. The histograms displayed are P2 control MSCs cultured in DMEM-LG. All histograms have been adjusted for height. b) Representative histograms from P0 cells in DMEM and ES medium. CD146 expression was significantly lower in ES than in DMEM in P0. c) Exemplary histograms of P2 control vs. FACS-sorted MSCs. d) Exemplary histograms of P2 control vs. MACS-sorted MSCs. There was an important variation of CD146 expression between patients, as demonstrated by the P2 samples from c and d. Magnetic sorting resulted in a lower purification than fluorescence sorting, while higher CD146 expression in naïve MSCs resulted in a higher purification result.