Table 1 List of primers used in the real-time PCR analysis of gene expression in C3H10T1/2 cells
Primer ID | Primers (5′-3′) | Amplicon size (bp) | Accession No. |
|---|---|---|---|
Aggrecan–F | AGTGGATCGGTCTGAATGACAGG | 105 | NM007424 |
Aggrecan–R | TTG GCA GCG TTC ATG TCG TAA | ||
Collagen type I–F | ATGCCGCGACCTCAAGATG | 153 | NM007742 |
Collagen type I–R | TGAGGCACAGACGGCTGAGTA | ||
Collagen type II–F | AGGGCAACAGCAGGTTCACATAC | 171 | NM031163 |
Collagen type II–R | TGTCCACACCAAATTCCTGTTCA | ||
SOX9-F | CAGTACCCGCATCTGCAC | 81 | NM011448 |
SOX9-R | TCTCTTCTCGCTCTCGTT | ||
Osteocalcin-F | CTCTGTCTCTCTGACCTCACAG | 133 | NM031368 |
Osteocalcin -R | GGAGCTGCTGTGACATCCATAC | ||
GAPDH-F | TGTGTCCGTCGTGGATCTGA | 150 | NM001001303 |
GAPDH-R | TTGCTGTTGAAGTCGCAGGAG |