Figure 3

Micro-CT and histomorphometric analysis of the contralateral proximal tibia 14 days post injection of mBSA. Bone turnover was measured at a location distant to the site of active inflammation to assess the systemic effects of joint inflammation. (A) Micro-CT. The bone volume fraction (bone volume/tissue volume (BV/TV]), trabecular number (Tb.N), trabecular separation (Tb.Sp), and trabecular thickness (Tb.Th) are shown for bones harvested. Bars show the mean ± SEM. Findings between the 4 groups were compared using the Mann–Whitney-Test with adjustment for 4 parallel tests (α* = 0.0125). NS = not significant (see Figure 1 for other definitions). (B) Histomorphometric quantification. Bone resorption, shown as osteoclast surface/bone surface (Oc.S/BS) and osteoclast number/bone surface (N.Oc/BS), respectively. Bone formation, shown as osteoblast surface/bone surface (Ob.S/BS). Bars show the mean ± SEM. Findings between the 4 groups were compared by using the Mann–Whitney test with adjustment for 4 parallel tests (α* = 0.0125). NS = not significant (see Figure 1 for other definitions).