Figure 2

Inhibition of IL-6, MMP-3, and CCL2 production from FLS by the selective CB ₂ agonist JWH133. CB₂ expression on in vitro cultured FLS from three RA patients was detected with Western blotting analysis (A). Bars indicate 50 and 37 kDa. Predicted molecular weight of CB₂ is 45 kDa. RA FLS were treated with various concentrations of JWH133, from 30 minutes before the stimulation with TNF-α for 24 h. Concentrations of IL-6 (B), MMP-3 (C), and CCL2 (D) in the cultured supernatants were measured with ELISA. Data is presented as means ± SEM of one of three independent experiments analyzed in quadruplicate. *p < 0.05, **p < 0.01.