Skip to main content

Table 1 Primer sequences and amplicon sizes for real-time RT-PCR

From: Osteogenic differentiation of amniotic epithelial cells: synergism of pulsed electromagnetic field and biochemical stimuli

Genes

Gene Bank ID

Primer Sequence (5'-3')

Amplicon (bp)

ALP

NM_000478

F: 5' GGAACTCCTGACCCTTGACC 3'

86

R: 5' TCCTGTTCAGCTCGTACTGC 3'

OC

NM_001199662

F:5' AGGGCAGCGAGGTAGTGAA 3'

151

R: 5' TCCTGAAAGCCGATGTGGT 3'

BMP-2

NM_001200

F:5' TCAAGCCAAACACAAACAGC 3'

103

R: 5' AGCCACAATCCAGTCATTCC 3'

RUNX2

NM_001015051

F:5' TTACTTACACCCCGCCAGTC 3'

139

R: 5' TATGGAGTGCTGCTGGTCTG 3'

β-catenin

NM_020248

F:5' CCTATGCAGGGGTGGTCAAC 3'

95

R: 5' CGACCTGGAAAACGCCATCA 3'

Integrinβ1

NM_002211

F:5' CAAGCAGGGCCAAATTGTGG 3'

121

R: 5' TGTCATCTGGAGGGCAACCC 3'

Nrf2

NM_001145413

F:5' ATTGCCTGTAAGTCCTGGTCA 3'

182

R: 5' ACTGCTCTTTGGACATCATTTCG 3'

Keap1

NM_203500

F:5' GTGGCTGTCCTCAATCGTCT 3'

127

R: 5' GGATGGTGTTCATTGCTGTG 3'

β-actin

NM_001099771

F:5' ACTATCGGCAATGAGCGGTTC 3'

77

R: 5' ATGCCACAGGATTCCATACCC 3'