Figure 5From: Osteogenic differentiation of amniotic epithelial cells: synergism of pulsed electromagnetic field and biochemical stimuli A marked increase of calcium deposits was evoked during the osteogenic differentiation of AECs. (A) Calcium deposition was assessed by alizarin red S staining (arrows) (magnification of 400×). (B) The amount of calcified deposition was semi-quantitatively calculated with ImageJ software. Experiments were performed three times with the similar results (n = 3 in each group). * indicates P < 0.05 vs Control, # indicates P < 0.05 vs PEMF and & indicates P < 0.05 vs OM.Back to article page