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Table 1 Forward and reverse primers and probes used for RT-PCR analysis

From: Dose-dependent regulation of cell proliferation and collagen degradation by estradiol on ligamentum flavum

Gene Primer/Probe Sequences
GADPH 5′-TTCATTGACCTCAACTACAT-3′
5′-GAGGGGCCATCCACAGTCTT-3′
Type I collagen 5′-CAGACCAACAACCCAAACTCAAT-3′
5′-TGCACTTTTGGTTTTTGGTCAC-3′
Elastin 5′-CCGCTAAGGCAGCCAAGTATGGA-3′
5′-AGCTCCAACCCCGTAAGTAGGAAT-3′
MMP-1 5′-CGGTTTTTCAAAGGGAATAAGTACT-3′
5′-TCAGAAAGAGCAGCATCGATATG-3′
MMP-2 5′-TGAGCTCCCGGAAAAGATTG-3′
5′-GGTGCTGGCTGAGTAGATCCA-3′
MMP-9 5′-CCTGGAGACCTGAGAACCAATC-3′
5′-TTCGACTCTCCACGCATCTCT-3′
MMP-13 5′-ACAGTTGATAGACTCCGAGAAATGC-3′
5′-ACCATTTGAGTGTTCGAGGGA-3′