Figure 2

Histological analysis of the fracture callus composition 14 and 28 days after surgery. Alcian blue staining was performed using sagittal section of the fractured femur. Using digital image analysis, the area of different tissues was determined within the whole callus as indicated in A. Overview of the tissue composition of a sagittal section of a fractured femur: Area of whole callus, black line; cartilage area, dotted line; soft tissue, dashed line. B: Comparison of the callus composition after 14 and 28 days with wild type, hTNFtg and hTNFtg + TNFi group within the site of fracture (AOI) after Alcian blue staining (representative pictures, scale bar 500 μm).