The mRNA expressions of the signaling molecules in the rats femoral heads of all groups. The expressions of TLR4, MyD88, NF-κB p65 and MCP-1 increased with time in the group B. Compared with the group N, there was a significant difference in the group B, but no significant in the group A. Between groups A and B, there were significant differences in the mRNA expressions of TLR4 and MCP-1 at each time point and NF-κB p65 at 12 weeks. (P < 0.05: # vs. group N; ★ vs. group A; P < 0.01: * vs. group N; Δ vs. group A).