The positive staining of the signaling molecules in the three groups by immunohistochemistry. Quantitative analysis demonstrated significant increase in immunolabeling as a percentage of total bone volume for the signaling molecules in the group B. Compared with the group N, the immunostaining for the signaling molecules in the group B increased significantly, but no significant in the group A. The expressions of TLR4 and MCP-1 in group B had significant differences compared with that in group A at each time point. MyD88 and NF-κB p65 expressions in group B had significant differences compared with that in group A at 12 weeks. (IOD: integrated optical density, P < 0.05: # vs. group N; ★ vs. group A; P < 0.01: * vs. group N; Δ vs. group A).