H&E staining and transmission electron microscopy of normal nerves and acellular nerves. H&E staining of the longitudinal sections of normal nerves (A) and acellular nerves (B). Following decellularization, H&E staining showed that the endoneurial tubes were empty. Transmission electron microscopy of cross-sections of normal nerves (C) and acellular nerves (D). TEM demonstrated that axons and myelin sheaths were absent in the acellular nerves. Scale bar = 20 μm (A, B), 1 μm (C, D).