TRAP expressing osteocytes. (A) A representative TRACP-stained section of the femur midshaft cortical bone demonstrates the greater number of TRACP + osteocytes in WT-Suspended mice compared to Control, with no difference seen in cKO-Suspended. (B) TRACP + osteocytes were quantified relative to total number of lacunae. The black bar represents 25 μm. Red arrow: TRACP + osteocyte, blue arrow: TRACP- osteocyte, white arrow: empty lacunae. Comparisons via two-way ANOVA. Mean ± SE. * indicates a significant difference (p < 0.05) between Control and Suspended within a genotype, while † indicates a significant difference (p < 0.05) between WT and cKO within a loading condition. n = 5/group.