Figure 4

Representative images of Live/Dead cell staining of MSCs cultured on BCP and nHA-coated BCP scaffolds. After 1 day in co-culture, living cells (stained green) had adhered to both scaffold types, exhibiting normal polygonal morphology (A, B), and few dead cells (stained red) were seen on either scaffold type (C, D). After co-culture for 14 days, MSCs had proliferated on both scaffold types. MSCs have not yet reached full confluence on most regions of the BCP scaffolds (E), but they had achieved a fully confluent monolayer on the nHA-coated BCP scaffolds (F). After 14 days in culture, few dead cells were seen on either scaffold type (G, H).