Figure 3

Histological observations of the ATDC5 cells cultured for 7 days using phase contrast microscopy (each scale bar shows 100 micrometer). The cells were cultured on PAMPS gel or PDMAAm gel in the maintenance medium without hyaluronic acid (HA) (“-“ shown in the photograph), and in the mediums containing HA of 0.01, 0.10, and 1.00 mg/mL concentrations. On the PAMPS gel, phase contrast microscopy did not show any nodules in each medium. On the PDMAAm gel, we found a tendency that the size of nodules was smaller in the mediums containing HA than in the medium without HA, and that the number of the nodules was less in the mediums containing HA of 1.00 mg/mL concentration than in the other medium with HA.