Expression of Sema3A, VEGF-A and NRP1 mRNA in synovial tissues. Expression levels of Sema3A, VEGF-A and NRP1 mRNA were measured using real-time PCR. The mRNA levels were normalized to the expression of β-actin. The box plots demonstrate the 10th and 90th percentile (whiskers), the 25th and 75th percentile, and the median. Sema3A mRNA levels were significantly decreased in RA (n = 30) synovial tissues compared with OA (n = 23) (A). VEGF-A and NRP1 mRNA levels were not significantly altered (B, C). Correlation of Sema3A mRNA levels and DAS28-CRP (D); VEGF-A mRNA levels and DAS28-CRP (E); and Sema3A/VEGF-A mRNA levels and DAS28-CRP (F). P values were obtained using the Mann–Whitney U-test. were examined using Spearman’s rank correlation coefficient.