Figure 5
From: Monocyte depletion increases local proliferation of macrophage subsets after skeletal muscle injury
CD68 + blood monocytes do not express CD163. Blood was isolated from uninjured rat. Total white blood cells were collected and submitted to antibody staining for flow cytometric analyses to assess CD163 expression on CD3-CD45RA-CD11b+CD68+ cells. Gates were set using omission of primary antibody for CD3/CD45RA and for CD11b; and using isotype control staining for CD68 and CD163. Results shown are representative of 4 individual observations.