Figure 3

Monocyte depletion induced significant increase in the number of proliferative macrophages in injured TA muscles. Rats were treated once a day with intravenous injection of liposome-encapsulated clodronate starting 24 h prior to injury until euthanasia at 2, 3 and 4 d post-injury for analysis of the number of proliferating Ki-67⁺M1 and Ki-67⁺M2 macrophages into injured muscle. (A) Total CD11b⁺CD68⁺ proliferating macrophages also positive for Ki-67⁺ (not shown) were identified. Gates were set using isotype controls for Ki-67 and CD68. (B) Ki-67⁺M1 and (C) Ki-67⁺M2 macrophage subsets were quantified at 2, 3 and 4 d post-injury. Liposome-encapsulated clodronate induced a significant increase of Ki-67⁺M1 and Ki-67⁺M2 macrophage numbers at 4 d post-injury. *Significantly different from non-depleted groups, Student’s t-test, p < 0.05. n = at least 4, for all the time points and conditions evaluated.