Figure 2
From: Monocyte depletion increases local proliferation of macrophage subsets after skeletal muscle injury
Numbers of M1 and M2 macrophages in TA muscle are modified by blood monocyte depletion. Rats were treated once a day with intravenous injection of liposome-encapsulated clodronate starting 24 h prior to injury until euthanasia at 1, 2, 3 and 4 d post-injury, for analysis of M1 and M2 macrophage accumulation into injured muscle. (A) Gating strategy (at 3 d post-injury) in single cell suspensions from skeletal muscles was based on omission of primary antibody for CD11b, and on isotype control stainings for CD68 and CD163 to identify M1 (CD163-) and M2 (CD163+) subsets. Liposome-encapsulated clodronate induced a significant decrease in accumulation of M1 (B) and M2 (C) macrophages at 2 d post-injury and a significant increase in M1 macrophage number at 4 d post-injury. *Significantly different from non-depleted groups, Student’s t-test, p < 0.05. n = at least 3, for all the time points and conditions evaluated.