Biochemical analysis of glycosaminoglycans in D21 scaffold constructs. Mean GAG/DNA (μg/μg) levels from a total of four independent scaffold culture experiments performed in duplicate. MFCs isolated from inner and outer human meniscus were seeded on porous collagen scaffolds and chondrogenically stimulated under normal (21% O2, NRX) or low (3% O2, HYP) oxygen tension. Data represents mean ± standard error of the mean. * = p <0.05 using one-way ANOVA with Tukey’s multiple comparisons post-test.