Figure 8From: Matrix forming characteristics of inner and outer human meniscus cells on 3D collagen scaffolds under normal and low oxygen tensions Deposition of sulfated proteoglycan in D21 constructs. Inner and outer MFC were cultured on collagen scaffolds under normal (21%) or low (3%) oxygen tension for 21 days. 5 μm paraffin-embedded sections were stained with Safranin-O and counterstained with fast green. Images captured from constructs seeded with inner and outer MFC cultured under normal (21% O2, top row) or low oxygen tension (3% O2, bottom row). Scale bar 100 μm. Asterisk represents scaffold collagen fiber and surrounding area where fibers have been replaced by ECM synthesized de novo.Back to article page