Figure 7
Immunohistochemical staining for collagen II at D21. Paraffin embedded sections of day 21 (D21) scaffold culture constructs were sectioned at 5 μm and the deposition of collagen type II was visualized by indirect immunohistochemistry in conjunction with streptavidin-horseradish peroxidase linked secondary antibody. Representative images of collagen type II distribution were captured from inner and outer MFCs cultured in 21% O2 and 3% O2 (scale bar 100 μm). Asterisk represents scaffold collagen fiber and surrounding area where fibers have been replaced by ECM synthesized de novo.