Figure 6From: Matrix forming characteristics of inner and outer human meniscus cells on 3D collagen scaffolds under normal and low oxygen tensions Gene expression levels of D21 scaffolds cultured under normal versus low oxygen tension. Inner and outer MFCs were expanded to P3 then chondrogenically stimulated in scaffold culture under normal (21%, white bars) or low (3%, black bars) O2 in vitro for 21 days (D21). Data represents the mean ± SEM of pooled values for inner and outer MFCs cultured under 21% compared to those cultured under 3% oxygen tension. Expression levels were normalized to β-actin using 2-ΔCT method. * = p < 0.05 using Student’s two-tailed t-test.Back to article page