Angiogenesis assay. After rADSCs were differentiated in endothelial differentiation medium for 8 days, the differentiated cells were placed in Matrigel in a 24-well plate. A: 1 hour after cell-seeding; B: 24 hours after seeding, exhibit partial tubule formation, C: 48 hours after cell seeding, clearly demonstrated capillary-like networks between cells. D: Western blot analysis using anti-vWF revealed up-regulation of vWF in rADSCs-Endo group. Bars indicate 100 μm.