Subchondral pre-solidified chitosan/blood implants elicit reproducible early osteochondral wound-repair responses including neutrophil and stromal cell chemotaxis, bone resorption and repair, enhanced repair tissue integration and delayed matrix deposition

Table 1 Chitosans and chitosan solutions used in this study

Chitosan	DDA (%)	M _w(kDa)	M _n(kDa)	PDI	Characteristics
High (150K) Powder*	81.8	274.6	241.2	1.1
High (150K) Solution**	81.8	124.2	107.4	1.2	pH=3.10 Osm=48mosm
RITC-150K ***	80.6	246.4	176	1.4	0.50% mol/mol RITC/chi
Low (40K) Powder*	81.9	47.7	37.3	1.3
Low (40K) Solution**	81.9	31.6	25.3	1.2	pH=1.65 Osm=72mosm
RITC-40K ***	80.6	61.5	46.7	1.3	0.52% mol/mol RITC/chi
Ultralow (10K) Powder*	81.9	22.8	10.3	2.2
Ultralow (10K) Solution**	81.9	23.8	12.7	1.9	pH=5.58 Osm=48mosm
RITC-10K ***	80.0	22.4	12.4	1.8	0.58% mol/mol RITC/chi

* Chitosan powder used to make the 20 mg/mL autoclave-sterilized solution; ** Autoclaved 20 mg/mL chitosan solution; *** RITC-chitosan powder used to make the 5 mg/mL filter-sterilized solution; DDA: Degree of deacetylation; M _w: Weight-average molecular weight; M _n: Number-average molecular weight; PDI: Polydispersity index (M _w/M _n); Osm: Osmolality; chi: chitosan.

ISSN: 1471-2474