Table 1 The primer sequence, product size and annealing temperature of target genes for qRT-PCR
Gene | Primer nucleotide sequence | Product size (bp) | Annealing temperature | Accession no. |
|---|---|---|---|---|
β-actin | 5′-ATCGTGGGCCGCCCTAGGCA-3′ (forward) | 243 | 52 | NM_031144 |
5′-TGGCCTTAGGGTTCAGAGGGG-3′ (reverse) | ||||
Bmp2 | 5′-TAGTGACTTTTGGCCACGACG-3′ (forward) | 81 | 58 | NM_017178 |
5′-GCTTCCGCTGTTTGTGTTTG-3′ (reverse) | ||||
Bmp4 | 5′-CATGGCTCGCGCCTCCTAGC-3′ (forward) | 184 | 58 | NM_012827 |
5′- ATTCCGAGCGACGCACTGCC-3′ (reverse) | ||||
Bmp7 | 5′- CAACCTAGTGGAGCACGACAAGGA-3′ (reverse) | 213 | 60 | NM_001191856 |
5′- AGGTCGGACTCCCTGCCTGAGT-3′ (reverse) | ||||
Bmpr1a | 5′-GCCACCCTGGACACCAGAGC-3′ (forward) | 101 | 60 | NM_030849 |
5′-GCAGGCTTGCCTTGCGTG-3′ (reverse) | ||||
Bmpr1b | 5′-CACCACGGAGGAAGCCAGC-3′ (forward) | 239 | 60 | NM_001024259 |
5′-GGCACAGGCCGCTGACAGAC-3′ (reverse) | ||||
Bmpr2 | 5′-GGTGCTGGTCTCACATTG-3′ (forward) | 180 | 58 | NM_080407 |
5′-GAGGCGGACTGAGTGGTG-3′ (reverse) |