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Table 1 The primer sequence, product size and annealing temperature of target genes for qRT-PCR

From: Higher BMP/Smad sensitivity of tendon-derived stem cells (TDSCs) isolated from the collagenase-induced tendon injury model: possible mechanism for their altered fate in vitro

Gene Primer nucleotide sequence Product size (bp) Annealing temperature Accession no.
β-actin 5′-ATCGTGGGCCGCCCTAGGCA-3′ (forward) 243 52 NM_031144
5′-TGGCCTTAGGGTTCAGAGGGG-3′ (reverse)
Bmp2 5′-TAGTGACTTTTGGCCACGACG-3′ (forward) 81 58 NM_017178
5′-GCTTCCGCTGTTTGTGTTTG-3′ (reverse)
Bmp4 5′-CATGGCTCGCGCCTCCTAGC-3′ (forward) 184 58 NM_012827
5′- ATTCCGAGCGACGCACTGCC-3′ (reverse)
Bmp7 5′- CAACCTAGTGGAGCACGACAAGGA-3′ (reverse) 213 60 NM_001191856
5′- AGGTCGGACTCCCTGCCTGAGT-3′ (reverse)
Bmpr1a 5′-GCCACCCTGGACACCAGAGC-3′ (forward) 101 60 NM_030849
5′-GCAGGCTTGCCTTGCGTG-3′ (reverse)
Bmpr1b 5′-CACCACGGAGGAAGCCAGC-3′ (forward) 239 60 NM_001024259
5′-GGCACAGGCCGCTGACAGAC-3′ (reverse)
Bmpr2 5′-GGTGCTGGTCTCACATTG-3′ (forward) 180 58 NM_080407
5′-GAGGCGGACTGAGTGGTG-3′ (reverse)