Skip to main content

Table 1 Primer sequences used in quantitative real-time PCR (all primers were purchased from Invitrogen, Mississauga, Ontario, Canada)

From: Effect of interleukin-1β treatment on co-cultures of human meniscus cells and bone marrow mesenchymal stromal cells

Gene Primer Direction
β-Actin 5′-AAGCCACCCCACTTCTCTCTAA-3′ (Forward)
5′-AATGCTATCACCTCCCCTGTGT-3′ (Reverse)
Aggrecan 5′-AGGGCGAGTGGAATGATGTT-3′ (Forward)
5′-GGTGGCTGTGCCCTTTTTAC-3′ (Reverse)
Collagen I (COL1A2) 5′-TTGCCCAAAGTTGTCCTCTTCT-3′ (Forward)
5′-AGCTTCTGTGGAACCATGGAA-3′ (Reverse)
Collagen II (COL2A1) 5′-CTGCAAAATAAAATCTCGGTGTTCT-3′ (Forward)
5′-GGGCATTTGACTCACACCAGT-3′ (Reverse)
Collagen 10A1 (COL10A1) 5′-GCCTCACTTATTAAAGCACAAAATGT-3′ (Forward)
5′-AATGGTTGAGAACAGCAAATTGC-3′ (Reverse)
IL1Ra 5′-CTGCACAGCGATGGAAGCT-3′ (Forward)
5′-GCCTTCGTCAGGCATATTGG-3′ (Reverse)
MMP-1 5′-ATGAGTCTTTGCCGGAGGAA-3′ (Forward)
5′-GTGACACCAGTGACTGCACATG-3′ (Reverse)
MMP-3 5′-CATCCAAAAACGCCAGACAA-3′ (Forward)
5′-CGGAGACTGGTAATGGCATCA-3′ (Reverse)
MMP-13 5′-CATCCAAAAACGCCAGACAA-3′ (Forward)
5′-CGGAGACTGGTAATGGCATCA-3′ (Reverse)
SOX9 5′-CTTTGGTTTGTGTTCGTGTTTTG-3′ (Forward)
  5′-AGAGAAAGAAAAAGGGAAAGGTAAGTTT-3′ (Reverse)