Accumulation of cartilaginous matrix in cell pellets after a total of 17 days culture in serum-free chondrogenic media. (A) Accumulated glycosaminoglycan (GAG) matrix content of cell pellets normalized to DNA amount. Data are mean ± standard error of mean (SEM) of 4 donors. (B) Comparison of experimental and calculated GAG per DNA contents of cells pellets. Calculated GAG per DNA content was calculated as per equation given in Methods and Materials sub-section Biochemical analysis. Data are mean ± standard error of mean (SEM) of 4 donor pairs. One-way analysis of variance (ANOVA) with Tukey’s multiple comparison post-tests: (*) indicates p <0.05, (**) indicates p < 0.001 and (***) indicates p < 0.0001 for statistical significance of comparison.