Effect of blockade of ERK or PI3K on the expressions of angiogenic makers. Cells overexpressing Foxc2 were cultured in osteogenic induction medium in the absence or presence of PD98059 (A, B, C) or LY294002 (D, E, F). Western blot results of VEGF or PDGF-β expression were indicated by the ratio of band intensity of VEGF or PDGF-β with β-actin (A, B, D, E). The results were expressed as mean±SD of triplicate experiments. Real time-PCR analysis of VEGF or PDGF-β was performed (C, F). The relative mRNA level of each gene was quantitated and statistically analyzed. *p<0.05 vs. control; #p<0.01 vs. control.