The effect of Foxc2 on osteogenic differentiation. (A, B and C) BMSCs were transfected with Lv-GFP or Lv-Foxc2 for 2 weeks. Cells stained with Alizarin red S (A and B, a1 presents the Lv-Foxc2 group, and a2 presents the Lv-GFP group). Cells stained with ALP (C, c1 presents the Lv-Foxc2 group and c2 presents the Lv-GFP group). (D) ALP activities in BMSCs. (E) Expressions of CON and Runx2 mRNA detected by real-time PCR 72 hours after transfection. The relative density of each gene was statistically analyzed. #p<0.01 vs. control. (F and G) CON and Runx2 expressions examined by Western blot 72 hours after transfection. The results were indicated by the ratio of band intensity of CON or Runx2 with β-actin. (H) Immunostaining for OCN and Runx2 (Texas-Red) with nuclear counterstained (DAPI-blue) 2 weeks after transfection (× 400). The results were expressed as mean±SD of triplicate experiments. #p<0.01 vs. control.