Figure 2
From: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
Expression of GFP and Foxc2 genes after transfection. (A) Transfection efficiency at various MOIs (10, 50, 100 and 200) 72 hours after transfection (×100). (B) Cell proliferation after transfection in the Lv-Foxc2 and Lv-GFP groups detected using MTT assay. (C) Foxc2 gene expression measured by real-time PCR 72 hours after transfection. GAPDH was used as a housekeeping gene. (D) Foxc2 protein expression evaluated by western blot 72 hours after transfection. Results were expressed as mean±SD of triplicate experiments. #p<0.01 vs. control.