Staphylococcus aureus biofilms decrease osteoblast viability, inhibits osteogenic differentiation, and increases bone resorption in vitro

Table 2 Relative expression of genes involved in osteogenic differentiation

	Day 7		Day 14
	Relative expression	Fold difference^a	Relative expression	Fold difference^a
Transcription factors
*runx2*
No Treatment (+ osteogenic media)*	1.12 ± 0.10	5.333	2.55 ± 0.23	3.643
UAMS-1	0.12 ± 0.18	0.590	0.93 ± 0.22	1.329
SAMMC-700	0.08 ± 0.25	0.381	0.13 ± 0.80	0.186
*atf4*
No Treatment (+ osteogenic media)*	35.4 ± 10.0	2.476	72.1 ± 0.30	3.433
UAMS-1	16.1 ± 0.15	1.126	26.1 ± 1.00	1.243
SAMMC-700	13.5 ± 0.16	0.944	19.3 ± 0.21	0.919
Genes involved in mineralization
*alp*
No Treatment (+ osteogenic media)*	5.73 ± 0.14	6.374	9.01 ± 0.04	15.615
UAMS-1	0.17 ± 0.01	0.187	1.12 ± 0.04	2.166
SAMMC-700	0.27 ± 0.01	0.306	0.21 ± 0.10	0.406
*bglap*
No Treatment (+ osteogenic media)*	0.77 ± 0.04	6.814	6.0 ± 0.33	40.816
UAMS-1	0.001 ± 1.00	0.009	0.10 ± 0.33	0.680
SAMMC-700	0.001 ± 0.51	0.009	0.01 ± 0.34	0.068
*sparc*
No Treatment (+ osteogenic media)*	174 ± 37.8	2.568	124 ± 0.21	1.879
UAMS-1	44.5 ± 22.7	0.656	48.7 ± 0.24	0.738
SAMMC-700	35.7 ± 24.2	0.526	84.9 ± 0.25	1.286

^afold difference is the difference in relative gene expression compared to osteoblasts grown under. non-osteogenic conditions (− osteogenic media).
*Indicates a positive osteogenic differentiation control; osteogenic media no BCM treatment.

ISSN: 1471-2474