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Table 1 Primers used in this study a

From: Staphylococcus aureus biofilms decrease osteoblast viability, inhibits osteogenic differentiation, and increases bone resorption in vitro

Gene

Accession number

Primer sequence

Activating transcription factor 4

ATF

Sense CCCTTCACCTTCTTACAACCTC

 

(NM_182810)

Antisense TGCCCAGCTCTAAACTAAAGGA

Runt-related transcription factor 2

RUNX2

Sense TGGTTACTGTCATGGCGGGTA

 

(NM_001015051)

Antisense TCTCAGATCGTTGAACCTTGCTA

Alkaline phosphatase

ALP

Sense AACATCAGGGACATTGACGTG

 

(NM_001127501)

Antisense GTATCTCGGTTTGAAGCTCTTCC

Osteocalcin

BGLAP

Sense CACTCCTCGCCCTATTGGC

 

(NM_000711)

Antisense GCCTGGGTCTCTTCACTACCT

Osteonectin

SPARC

Sense CCCATTGGCGAGTTTGAGAAG

 

(NM_003118)

Antisense AGGAAGAGTCGAAGGTCTTGTT

Receptor Activator of NF-KB Ligand

RANK-L

Sense GTCTGCAGCGTCGCCCTGTT

 

(NM_003701)

Antisense ACCATGAGCCATCCACCATCGC

Osteoprotegerin

OPG

Sense CGCCTCCAAGCCCCTGAGGT

 

(NM_002546)

Antisense CAAGGGGCGCACACGGTCTT

Glyceraldehyde-3-phosphate

GAPDH

Sense CAGCCTCCCGCTTCGCTCTC

 

(NM_002046)

Antisense CCAGGCGCCCAATACGACCA

  1. aPrimers used in this study were obtained from Primer Bank “PCR Primers for gene Expression Detection and Quantification”. http://pga.mgh.harvard.edu/primerbank/ (The Center for Computational and Integrative Biology). All selections were made relative to comparable annealing temperatures, as well as products close to 100bp in length to maintain consistent PCR conditions.