Figure 8
A,B. Double-staining tachykinin/betaIII-tubulin. Parts of focal myositis area of experimental animal (1w group, exercised side, gastrocnemius muscle). Staining for tachykinin (A) and for betaIII-tubulin (B). Tachykinin-like immunoreactivities show co-localization with betaIII-tubulin immunoreactivities (arrows). These reactions are thus nerve fiber related. Tachykinin-like immunoreactivity, but not betaIII-tubulin immunoreactivity, also occur for blood vessel walls (arrowheads). Magnification x200. c,d. Double-labelling tachykinin/S-100beta. Parts of focal myositis area of experimental animal (1w group, exercised side, gastrocnemius muscle). Tachykinin-like immunoreactivities occuring in groups are observable (arrowheads, C). They are associated with S-100beta immunoreactive cells (arrowheads, D). Please note that the nuclei of the cells show a pink/bluish colur reaction (combination of DAPI + S100beta reactions). Nuclei associated with muscle fiber (MF) show the characteristic blue DAPI reaction (curved arrows). Magnification x200.