Figure 11

Tachykinin-like immunoreactivity in blood vessel walls. Stainings with antibody sc-14104 are shown; gastrocnemius muscle specimens of the 1w group (exercised side) (A-C, inset C), and soleus muscle specimen from 6w (D) group (non-exercised side). The region shown in (A) is a region located adjacent to a focal myositis area. Large arrows point at blood vessel reactions (A-D). There are varying degrees of tachykinin-like immunoreactivity in blood vessel walls in (A). Vessel walls of the 1w specimens show strong (B) (focal myositis area) and moderate (C) (vessel in normally appearing area) reactions. A high intensity of immunolabelling is seen in the walls in (D). The immunoreactivity is in principle confined to the smooth muscle layers; very weak or neglible reactions are seen in the endothelial layer (small arrows B,D). In the inset in (C), it is obvious that the specific reaction is present in the smooth muscle layer (green colour) (large arrow) whilst the endothelial part is non-reactive (small arrow). In this inset, the DAPI staining of the nuclei is visible. Magnification x50 (A), x200 (B-D).