Figure 4

Immunohistochemistry staining and species specific genomic in situ hybridization of an implanted hydrogel upon harvest. A: Detection of collagen type I; B: collagen type II; C: aggrecan by immunohistochemistry. The blue dots are the DAPI stained nuclei. Note the even distribution of the nuclei across the samples, except for the edge in A. Species specific in situ hybridization discriminates between cells of human origin (D) and cells of murine origin (E). Mouse cells are predominantly found at the edge of the xenotransplant, human cells are distributed evenly within the transplant.