Table 1 Primers used for RT-PCR
Abbreviation | Gene description | forward primer | reverse primer | |
|---|---|---|---|---|
Reference gene | 18S | Reference gene 18S ribosomal RNA | AGT GCG GGT CAT AAG CTT GC | GGT GTG TAC AAA GGG CAG GG |
ACAN | Aggrecan | GAG GTC GTG GTG AAA GGT GT | GTG TGG ATG GGG TAC CTG AC | |
Anabolic | Col1 | Collagen type 1 | TTC TTG GTG CTC CTG GCA TTC | GCA ATC CGT TGT GTC CCT TTA TG |
Col2 | Collagen type 2 | GAC CCC ATG CAG TAC ATG CG | CCA GTA GTC ACC GCT CTT CC | |
MMP-1 | Metalloproteinase-1 | ATA CCT GGA AAA CTA CTA CA ATC TG | TCT TCA GGG TT TCA GCA TCT | |
Catabolic | MMP-3 | Metalloproteinase-3 | AGC CAA TGG AAA TGA AAA CTC TTC | CCA GTG GAT AGG CTG AGC AAA |
MMP-13 | Metalloproteinase-13 | TGC CCC TCC TCA ACA GTA AC | GAG CCC GCT GCA TTC TTC TT | |
TIMP1 | Tissue inhibitor of metalloproteinase 1 | AGC AGA GCC TGC ACC TGT GT | CCA CAA ACT TGG CCC TGA TG | |
TIMP3 | Tissue inhibitor of metalloproteinase 3 | TCT GCA ACT CCG ACA TCG TG | CGG ATG CAG GCG TAG TGT T | |
ADAMTS4 | A disintegrin and metalloproteinase with thrombospondin motifs 4 | GAC CTT CCG TGA AGA GCA GTG T | CCT GGC AGG TGA GTT TGC AT | |
TNFα | Tumor necrosis factor alpha | CAG CCT CTT CTC TTT CCT GCT | CCG ATC ACC CTG AAG TGC | |
Inflammatory | IL1b | Interleukin-1beta | TCC AGA CGA GGG CAT CCA | CTG CCG GAA GCT CTT GTT G |
IL-6 | Interleukin-6 | CTG GTG GTG GCT ACC GCT TT | ATG GTC TCC AGG ATG CTC CG | |
IL-8 | Interleukin-8 | CAA CCT TCC TGC TGT CTC TG | GGT CCA CTC TCA ATC ACT CT |