Muscle contents of myogenin, myoD, pax7, and myostatin as determined by Western blotting. The expression of myogenin before and after LC-induced muscle injury was examined. Obtained bands and their quantifications are shown (A). The expression of myoD (B), pax7 (C), myostatin (D) were also examined. Details are described in the materials and methods section. **: p < 0.01, ***: p < 0.001, compared to pre-treatment levels. Values are means and SDs.