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Table 1 Primers and restriction enzymes for PCR-RFLP analysis

From: A single-nucleotide polymorphism rs708567 in the IL-17RC gene is associated with a susceptibility to and the curve severity of adolescent idiopathic scoliosis in a Chinese Han population: a case-control study

SNP Primers Annealing Temperature (°C) PCR Product Size (bp) Restriction Enzyme Fragment Size After Enzymatic Cleavage (bp)
rs708567 Forward Primer: 5’-AGTAGGGTAGGCCTGGAAGG-3’ 57 210 HinfI GG: 210
  Reverse Primer: 5’-CACTGGGAAGAGCCTGAAGA-3’     AG: 161 + 49
  1. *PCR-RFLP, polymerase chain reaction restriction fragment length polymorphism; SNP, single-nucleotide polymorphism.