Table 1 Target sequences and corresponding oligonucleotide sequences for the four shRNAs, designated shRANK-1, shRANK-2, shRANK-3 and scramble shRNA
From: Inhibition of osteoclastogenesis by RNA interference targeting RANK
shRNA | Target sequences | Oligonucleotide sequences |
|---|---|---|
shRANK-1 | caagaagtgtgtgaaggta | Forward: 5’- -3’ GATCCCCCAAGAAGTGTGTGAAGGTATTCAAGAGATACCTTCACACACTTCTTG TTTTTA |
Reverse:5’- -3’ AGCTTAAAAACAAGAAGTGTGTGAAGGTATCTCTTGAATACCTTCACACACTTCTTG GGG | ||
shRANK-2 | tgggcttcttctcagatgt | Forward: 5’- -3’ GATCCCCTGGGCTTCTTCTCAGATGTTTCAAGAGAACATCTGAGAAGAAGCCCA TTTTTA |
Reverse: 5’- -3’ AGCTTAAAAATGGGCTTCTTCTCAGATGTTCTCTTGAAACATCTGAGAAGAAGCCCA GGG | ||
shRANK-3 | gcgctgacagctaatttgt | Forward: 5’- -3’ GATCCCCGCGCTGACAGCTAATTTGTTTCAAGAGAACAAATTAGCTGTCAGCGC TTTTTA |
Reverse: 5’- -3’ AGCTTAAAAAGCGCTGACAGCTAATTTGTTCTCTTGAAACAAATTAGCTGTCAGCGC GGG | ||
scrambled shRNA | Forward: 5’- -3’ GATCCCCGCCAGCTTAGCACTGACTCTTCAAGAGAGAGTCAGTGCTAAGCTGGCTTTTT A | |
Reverse: 5’- -3’ AGCTTAAAAAGCCAGCTTAGCACTGACTCTCTCTTGAAGAGTCAGTGCTAAGCTGGCGGG |