Table 1 Primer sequences, conditions and annealing temperature of PCR assay for aggrecan mRNA amplification
From: Characterization of microRNA expression profiles in normal and osteoarthritic human chondrocytes
Primers | Sequence | Length | %GC | Annealing Temperature | Amplicon (bp) |
|---|---|---|---|---|---|
AGG 1 F | 5′ GCCTACGAAGCAGGCTATGA 3′ | 20 mer | 55 | 60°C | 136 |
AGG 1R | 5′ GCACGCCATAGGTCCTGA 3′ | 18 mer | 61 | ||
HPRT 1 F | 5′ TGACCTTGATTTATTTTGCATACC 3′ | 24 mer | 33 | 60°C | 102 |
HPRT 1R | 5′ CGAGCAAGACGTTCAGTCCT 3′ | 20 mer | 55 |