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Table 1 Primer sequences of BMP-2, PPAR-γ and GAPDH for RT-PCR

From: The effect of core decompression on local expression of BMP-2, PPAR-γ and bone regeneration in the steroid-induced femoral head osteonecrosis

Gene

Primer sequences

Length(bp) of product

Tm(°C)

Nucleotide number

BMP2

5' CGA AAC ACA AAC AGC GGA AAC 3'

97

84.2

AF041421

5' GCC ACA ATC CAG TCG TTC CA 3'

PPARγ

5' AGT CGC CAT CCG CAT CTT 3'

147

86.8

AF013266

5' ATC TCA TGG ACG CCG TAC TTG 3'

GAPDH

5' TGA CAA CGA ATT TGG CTA CAG 3'

83

83.6

L23961

5' GGT GGT TTG AGG GCT CTT ACT 3'