Effect of statin exposure on neurite outgrowth. Fluvastatin exposure for 96 h resulted in a marked degeneration of neurites growing out from organotypic spinal cord cultures, when plated on collagen coated membranes. Cultures were stained with SMI32 antibodies developed with FITC-conjugated secondary antibody as described in the text. Vigorous outgrowth of ventral horn neurites is apparent in panel A, montage view (bar equals 1 mm) and panel B, detail view (bar equals 500 μm). Neuritic degeneration is seen in panel C (bar equals 500 μm).