Figure 4

Time-course study of total PAI-1 protein secretion with dexamethasone and simvastatin. Human bone marrow adipocytes were cultured in serum-free DMEM, and divided into four groups. Cont.: untreated; DEX: treated with 1 μM dexamethasone at 12 h; SIM: treated with 10 μM simvastatin at 0 h; SIM+DEX: treated with 10 μM simvastatin at 0 h following 1 μM dexamethasone at 12 h. ELISA of total PAI-1 protein secretion in culture medium was performed at 12, 18, 24 and 36 h. Pre-treatment of simvastatin down-regulated total PAI-1 protein secretion induced by dexamethasone to control levels. Data represent total PAI-1 protein secretion levels (ng/mL), and are expressed as means ± SD. *P < 0.05 vs. untreated control cells at identical time points.