Figure 3

Effects of dexamethasone and simvastatin on total PAI-1 and adiponectin protein secretions after 36 h of suspension culture. Human bone marrow adipocytes were cultured in serum-free DMEM with 1 μM dexamethasone, 10 μM simvastatin or 10 μM simvastatin pretreatment following 1 μM dexamethasone at 12 h. ELISA analysis of total PAI-1 protein secretion (A) and adiponectin protein secretion (B) were performed after 36 h of suspension culture. Cont.: control group, DEX: dexamethasone-treated group, SIM: simvastatin-treated group, SIM+DEX: simvastatin following dexamethasone-treated group. Total PAI-1 secretion was up-regulated by dexamethasone and down-regulated by simvastatin. Simvastatin reversed the dexamethasone induced PAI-1 secretion below control levels. Dexamethasone and simvastatin slightly, but not significantly, increased adiponectin secretion. Data represent relative secretion levels vs. control levels and are expressed as means ± SD. *P < 0.05 between two values.