Table 1 The selected SNPs (position/function), designed primers and amplicon sizes.
Gene | SNP | SNP position* | Function | Primers Forward/Reverse | Amplicon size |
|---|---|---|---|---|---|
BMP-2 | rs1005464 | intron 2 c.347-2744 G > A | intron | 5'-TGAGCGTATATTCCCTAACC-3' 5'-TAACCTCCCAAAAAATTAAATGAC-3' | 378 bp |
| Â | rs235768 | exon 3 c.570 A > T | missense mutation (p.R190S) | 5'-GCAGAGCTTCAGGTTTTCCG-3' 5'-TGTTTCTCCTCCAAGTGGGC-3' | 269 bp |
| Â | rs235764 | intron 2 c.346+3126 G > A | intron | 5'-ACTGACATTTTCCGTTCCACCT-3' 5'-TAACAGACAACTGATCAAGGAG-3' | 305 bp |
BMP-7 | rs4811822 | intron 2 c.612-1290 C > T | intron | 5'-CCCAGGGCAACAACAGTCTC-3' 5'-CCTGGGCACACAACTTGACC-3' | 260 bp |
| Â | rs1475000 | intron 2 c.611+10288 G > A | intron | 5'-TGCAGATGCTGGGTCCTTAA-3' 5'-CGGGTCAGATGCCCATGAAG-3' | 281 bp |
| Â | rs186659 | intron 1 c.419-2863 G > A | intron | 5'-CTGCAGGGCCTCATACACTA-3' 5'-GAGAACAGCTTCCAGGGTGA-3' | 292 bp |
NOG | rs1442828 | intragenic g.13328730 A > G | intragenic | 5'-TCCTCTTCGGTCATCCAGTG-3' 5'-TGGTGGAAACCTTGCCATTC-3' | 199 bp |
| Â | rs1372857 | intragenic g.13334320 A > G | intragenic | 5'-CTGGGAGGGTTCTTGATTGG-3' 5'-ACATGTGAAATGCAGGGCAG-3' | 170 bp |
| Â | rs9915822 | intragenic g.13320012 G > T | intragenic | 5'-TTAGGCGTCACCCACAGTTG-3' 5'-TGGGCAAGGTAAATGGAAGC-3' | 190 bp |
SMAD6 | rs2053423 | intron 3 c.13320013 C > T | intron | 5'-CATGGCTTGGATGCTTGGTGT-3' 5'-TTCCCAGTCCAAATCAGGGT-3' | 398 bp |
| Â | rs2119261 | intron 3 c.952+3144 C > T | intron | 5'-GCCACTACTGGACAAACCTT-3' 5'-TCCAACAACTACTCGGCAGA-3' | 414 bp |
| Â | rs3934908 | intron 3 c.953-11868 C > T | intron | 5'-GAATTGGATGGAGACACGTACC-3' 5'-GATCTGGAATGCTTCCTGAG-3' | 542 bp |