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Table 1 The selected SNPs (position/function), designed primers and amplicon sizes.

From: Genetic predisposition to fracture non-union: a case control study of a preliminary single nucleotide polymorphisms analysis of the BMP pathway

Gene SNP SNP position* Function Primers
Forward/Reverse
Amplicon size
BMP-2 rs1005464 intron 2
c.347-2744
G > A
intron 5'-TGAGCGTATATTCCCTAACC-3'
5'-TAACCTCCCAAAAAATTAAATGAC-3'
378 bp
  rs235768 exon 3
c.570
A > T
missense
mutation
(p.R190S)
5'-GCAGAGCTTCAGGTTTTCCG-3'
5'-TGTTTCTCCTCCAAGTGGGC-3'
269 bp
  rs235764 intron 2
c.346+3126
G > A
intron 5'-ACTGACATTTTCCGTTCCACCT-3'
5'-TAACAGACAACTGATCAAGGAG-3'
305 bp
BMP-7 rs4811822 intron 2
c.612-1290
C > T
intron 5'-CCCAGGGCAACAACAGTCTC-3'
5'-CCTGGGCACACAACTTGACC-3'
260 bp
  rs1475000 intron 2
c.611+10288
G > A
intron 5'-TGCAGATGCTGGGTCCTTAA-3'
5'-CGGGTCAGATGCCCATGAAG-3'
281 bp
  rs186659 intron 1
c.419-2863
G > A
intron 5'-CTGCAGGGCCTCATACACTA-3'
5'-GAGAACAGCTTCCAGGGTGA-3'
292 bp
NOG rs1442828 intragenic
g.13328730
A > G
intragenic 5'-TCCTCTTCGGTCATCCAGTG-3'
5'-TGGTGGAAACCTTGCCATTC-3'
199 bp
  rs1372857 intragenic
g.13334320
A > G
intragenic 5'-CTGGGAGGGTTCTTGATTGG-3'
5'-ACATGTGAAATGCAGGGCAG-3'
170 bp
  rs9915822 intragenic
g.13320012 G > T
intragenic 5'-TTAGGCGTCACCCACAGTTG-3'
5'-TGGGCAAGGTAAATGGAAGC-3'
190 bp
SMAD6 rs2053423 intron 3
c.13320013
C > T
intron 5'-CATGGCTTGGATGCTTGGTGT-3'
5'-TTCCCAGTCCAAATCAGGGT-3'
398 bp
  rs2119261 intron 3
c.952+3144
C > T
intron 5'-GCCACTACTGGACAAACCTT-3'
5'-TCCAACAACTACTCGGCAGA-3'
414 bp
  rs3934908 intron 3
c.953-11868
C > T
intron 5'-GAATTGGATGGAGACACGTACC-3'
5'-GATCTGGAATGCTTCCTGAG-3'
542 bp
  1. (* SNP position according to NCBI SNP: Geneview)